Tribioscience - Buying Guide

Bestselling from Tribioscience on Amazon

1. Thiol Fluorometric Assay (TBS2026)
2. Fast Glucose Determination Assay (Colorimetric/Fluorometric) (TBS2087) (600)
3. WST-8 or Cell Count Kit-8 (CCK-8) (TBS2022) (5000)
4. XTT Cell Viability Assay (TBS2021) (1000)
5. Cytochrome C Oxidase Activity Assay (TBS2115)
6. Tribioscience Cytochrome C Reductase Activity Assay (TBS2116)
7. Caspase-1 Colorimetric Assay Kit (TBS2040)
8. Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay (TBS2102C)
9. Caspase-3 Fluorometric Assay kit (TBS2035)
10. Tribioscience Beta-N-Acetylglucosaminidase (Beta NAG) Activity Colorimetric Assay (TBS2105) (200)

Features

• Applications: Measure free thiol group in a variety of samples like cells and tissues.
• KIT COMPONENTS: [GSH standard; 400 M (10X) 80 L], [Thiol Fluorescent Probe (100X) 60 L], [Assay Buffer 10 mL]
• STORAGE AND SHELF-LIFE: Store at -20 C; Shelf-life: 1 year.
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Features

• Applications: Direct Assays: Glucose in serum, plasma, urine, and other bio-samples.
• Kit Contents: (for 100 Assays) Component: 5x Assay Buffer 1x 12mL, 3x 12mL; Red Probe 1x 0.25mL, 1x 0.8mL; Glucose Standard Stock (2mM) 1x 100 L, 1x 300 L; Enzymes 1x 2mL, 3x 2mL.
• Storage and Handling: Store kit at -20 . Shelf life of 6 months. Protect from light.
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Features

• Applications: Cell proliferation, Drug screening, Growth factors, Cytotoxicity, Apoptosis
• Kit Components: CCK-8 Reagent (5 mL 500 Assays) (10 mL 1000Assay) (50 mL 5000Assays)
• Storage Condition: 2-8 C; Shelf-life: 1 years
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Features

• STORAGE: Storage: -20 C; Shelf-life: 12 months
• APPLICATIONS: Cell proliferation, Drug reagents test, Growth factors, Cytotoxicity, Apoptosis
• Kit Components: XTT Reagent (25 mL) (2x 25mL), XTT Activator (0.5 mL) (1.0mL)
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Features

• Storage Conditions: The kit ships on wet ice and storage at 20 C is recommended. When stored unopened, the components in this kit are stable for 24 months. After the initial thawing of the 1 M Dithiothreitol Solution, divide the solution into undiluted working aliquots (still at 1 M concentration) and store at -20 C.
• Components: Assay Buffer 5x 5 mL; Enzyme Dilution Buffer 2x 2 mL; Cytochrome c Solution 1 mL; Dithiothreitol (DTT) Solution 30 L; n-Dodecyl -D-maltoside Buffer 2 mL; Cytochrom c Oxidase Positive Control 50 L.
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Features

• Applications: Measuring NADPH-cytochrome c reductase levels in biological samples after exposure to drugs or other xenobiotics. Detection of ER during isolation and subcellular fractionation by density gradient separation.
• Key Features: Flexible: The kit can be used for 96-well plates or 384-well plates. Accurate: Accurate detection of cytochrome C reductase activity in small size as 50 L samples. Simple: Just load-Read. The kit can be used for a robust method. Time-saving: Less than 30 minutes.
• Kit Contents: Assay Buffer 15 mL; Cytochrome C 1.2 mL; NADPH 2 mL; Cytochrome C Reductase Positive 0.25 mL; Cytochrome C Oxidase Inhibitor 1 mL
• Storage Conditions: Store kit at -20 C. Shelf life of 2 years. Protect from light. Ship with blue ice.
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Features

• APPLICATIONS: Apoptosis, Drug screening, Growth factors, Cytotoxicity
• Kit Components for 100 Assays: Cell Lysis Buffer-A 6 mL, Assay Buffer-B 6 mL, Substrate 120 L, DTT (1M) 0.6 mL.
• Storage for 100 Assays: Cell Lysis Buffer-A 4 , Assay Buffer-B 4 , Substrate -20 , DTT (1M) -20 . Shelf Life: 1year.
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Features

• Features: Flexible: Suitable for 96-well plate. Accuracy: Absorbance measurement is proportional to the G6PDH concentration. Sensitive: Can detect as low as 0.2 mU/mL. Time-saving: Just incubate and read out style.
• Applications: Measure G6PDH activity in a variety of samples like cells and tissues.
• Kit Components (100 tests): G6PDH Substrate 60 L, G6PDH Cofactor 20 L, G6PDH Colorimetric Detection Probe 1.2 mL, G6PDH Assay Buffer 10 mL, G6PDH Positive Control (10X) 30 L, NADH Standard (2 mM stock) 200 L.
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Features

• Applications: Apoptosis, Drug screening, Growth factors, Cytotoxicity.
• Kit Components 100 Assays: Cell Lysis Buffer-A 6 mL, Assay Buffer-B 6 mL, Substrate 120 L, DTT (1M) 0.3 mL.
• Storage for 100 Assays: Cell Lysis Buffer-A 4 , Assay Buffer-B 4 , Substrate -20 , DTT (1M) -20 , Shelf Life: 1year.
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Features

• Applications: This kit is used for the determination of hexosaminidase activity in biological samples.
• Key features: Fast and sensitive: Linear detection range (20 L sample): 0.05 to 50 U/L for a 30 minutes reaction at 37 C. High throughput: This can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
• Storage Conditions: This kit is shipped on ice. Store all components at -20 in a non-frost-free freezer. Shelf life of 12 months after receiving.
• Kit Contents: Component 100x RXNS 200x RXNS: Substrate 10 mL 20 mL, Standard (10mM) 1 mL 2 mL, Hexosaminidase positive control 50 L 100 L, Stop Reagent 12ml 24mL, Assay Buffer 12ml 24mL
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Features

• Applications: This kit is used for the determination of hexosaminidase activity in biological samples.
• Key features: Fast and sensitive: Linear detection range (20 L sample) for a 30 minutes reaction at 37 C. High throughput: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
• Storage Conditions: The kit is shipped on ice and should be stored at -20 C for long-term storage. Shelf life of 12 months after receipt.
• Kit Contents: Component 100x Rxns 200x Rxns, Substrate (100X) 100 L 200 L, Standard (1 mM) 50 L 100 L, NAG positive control 50 L 100 L, Stop Reagent 12 mL 24 mL, Assay Buffer 15 mL 30 mL
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Features

• Applications: This kit is used for the determination of hexosaminidase activity in biological samples.
• Key features: Fast and sensitive: Linear detection range (20 L sample): 0.05 to 50 U/L for a 30 minutes reaction at 37 C. High throughput: This can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
• Storage Conditions: This kit is shipped on ice. Store all components at -20 in a non-frost-free freezer. Shelf life of 12 months after receiving.
• Kit Contents: Component 100x RXNS 200x RXNS: Substrate 10 mL 20 mL, Standard (10mM) 1 mL 2 mL, Hexosaminidase positive control 50 L 100 L, Stop Reagent 12ml 24mL, Assay Buffer 12ml 24mL
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Features

• Applications: This kit is used for the determination of hexosaminidase activity in biological samples.
• Key features: Fast and sensitive: Linear detection range (20 L sample) for a 30 minutes reaction at 37 C. High throughput: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
• Storage Conditions: The kit is shipped on ice and should be stored at -20 C for long-term storage. Shelf life of 12 months after receipt.
• Kit Contents: Component 100x Rxns 200x Rxns, Substrate (100X) 100 L 200 L, Standard (1 mM) 50 L 100 L, NAG positive control 50 L 100 L, Stop Reagent 12 mL 24 mL, Assay Buffer 15 mL 30 mL
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Features

• Applications: Direct Assays: as low as 1 M of ADP in cells and other biological samples.
• Key Features: Sensitive and Accurate: Use 10 L samples. Detection range 1-1000 M in 96-well plate assay. Simple and High-Throughput: Simple procedure; takes less than 30 minutes. Kit is designed to be a robust method.
• Storage Conditions: Store kit at -20 . Shelf life of three months. Except for Enzyme warm all of the components to room temperature before use. Briefly centrifuge all small vials prior to opening.
• KIT CONTENTS: Assay buffer 24 mL, Probe 120uL, Substrate 120uL, Enzyme 600uL, ADP standard 100uL 50mM
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Features

• Applications: Direct Assays: as low as 0.5 M of ATP in cells and other biological samples. Assay of enzymes that produce or degrade ATP.
• Key Features: Sensitive and accurate: Use 10 L samples. Detection range 0.5-1000 M in 96-well plate assay. Simple and high-throughput: Simple procedure; takes less than 30 minutes. The kit is designed to be a robust method.
• Storage and Handling: Store kit at -20 C. Shelf life of three months. Except Enzyme warm all of the components to room temperature before use. Briefly centrifuge all small vials prior to opening.
• KIT CONTENTS: Assay buffer: 24 mL; Probe: 120 uL; Substrate: 120 uL; Enzyme: 240 uL; ATP standard(50mM): 100uL.
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Features

• Features and Benefits: Accurate and consistent DNA extraction from mouse tail.
• Fast: Only 10 minutes for the whole process.
• Instant use: No need for additional materials
• Simple and safe procedure
• No use of organic solvents
• Ready for use in PCR and other enzymatic reactions
• Storage Conditions: Store kit at -20 C.
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Features

• Key Features: Convenient: The system contains all components for DNA extraction and PCR amplification. No Loading Dye Required: The PCR products can be directly loaded onto electrophoresis gel. High Fidelity: The optimal buffer condition and specifically engineered Taq DNA polymerase have increased the efficiency of PCR amplifications.
• Kit Contents: Fast DNA Extraction: 10mL, 2x PCR Ready Mix: 2x 1.25mL, Sufficient reagent for 250 x 20 L
• Storage Conditions: The kit is shipped on ice. Store all components at -20 C in a non-frost-free freezer. Shelf life is 12 months after receiving.
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Features

• Applications: Cell culture supernatants, cell lysates, or other tryptase containing supplies. Testing of purified tryptase enzyme, in vitro inhibitor screening. Cell degranulation
• KIT CONTENTS (100 assay): 5x Assay Buffer 10 mL, Tryptase inhibitor 200 L, Tryptase Substrate 2 mL, pNA Standard 100 L (10 mM), Calcium Ionophore 50 L(1mM), Tryptase Positive Control 200 L (10 g/ mL)
• Key Features: Sensitive and Accurate: The kit detects as low as 10 M in solution. Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.
• Storage Conditions: Store the kit at -20 protected from life Shelf life: 6 months.
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Features

• Typical Data: This standard curve(R^2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
• Sensitivity: The minimum detectable dose (MOD) of human is typically 200 pg/ml. The Intra-assay CV and the Inter-assay CV are 10%.
• Specificity: This assay recognizes natural and recombinant human pTau-231. No cross-reactivity with others.
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Features

• KIT CONTENTS FOR 100 TESTS: Name Size (100 tests); NNMT Substrate SAM 60 L; NNMT Substrate NA 40 L; NNMT Assay Buffer 12 mL; Thiol Detecting Probe (80X) 80 L; Enzyme mix II 2500 L; Enzyme I 40 L; 1-Methylnicotinamide (MNA, 100 mM) 50 L
• Storage conditions: Store the Reagent at 20 C protected from light. Shelf life: 12 months. For Enzyme I and Enzyme II, long term storage at 80 C.
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Features

• Typical Data: This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
• Sensitivity: The minimum detectable dose (MOD) of human is typically 200 pg/ml. The Intra-assay CV and the Inter-assay CV are 10%.
• Specificity: This assay recognizes natural and recombinant human pTau-217. No cross-reactivity with others.
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Features

• Application: Measure NNMT activity in a variety of samples.
• Storage conditions: Store the Reagent at 20 ( 80 C for enzyme NNMT and SAH Enzyme Mix), protected from light.
• Shelf life: 6 months.
• KIT CONTENTS FOR 100 TESTS: NNMT Substrate SAM 60 L; NNMT Substrate NA 30 L; NNMT Assay Buffer 20 mL; Thiol Probe (100X) 60 L; SAH Enzyme Mix 2500 L; NNMT positive control 50 L; GSH stock (400 M) 80 L
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Features

• Typical Data: This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
• Sensitivity: The minimum detectable dose (MOD) of human is typically 200 pg/ml. The Intra-assay CV and the Inter-assay CV are 10%.
• Specificity: This assay recognizes natural and recombinant human pTau-181. No cross-reactivity with others.
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Features

• Key Features Convenient: 2x Genotyping PCR Ready Mix contains all components for PCR amplification. All you need to do is to add primers, templates, and water for the PCR reaction. Hazard-free Visualization: Dyes are non-toxic and non-mutagenic. No Loading Dye Required: The PCR products can be directly loaded onto the electrophoresis gel. High efficiency: The optimal buffer condition and specifically engineered Taq DNA polymerase have increased the efficiency of PCR amplification.
• Kit Contents 2x 1.25 mL of 2x PCR Ready Mix 2x 1.25 mL of DNase free water. Sufficient reagent for 250 x 20 L Storage Conditions The kit is shipped on ice. All components should be stored at -20 C in a non-frost-free freezer. Shelf life is 12 months after receiving.
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